Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Mouse Pyruvate Dehydrogenase Protein X Component, Mitochondrial, PDHX ELISA Kit

Mouse Pyruvate Dehydrogenase Protein X Component, Mitochondrial, PDHX ELISA Kit

Mouse Pyruvate Dehydrogenase Protein X Component, Mitochondrial, PDHX ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.33ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 7.33ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Pyruvate dehydrogenase protein X component, mitochondrial

Code

E2416Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.33ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex (By similarity).

UniProt accession

MASS(Da)

53,999

GeneID

27402

Synonyms

AI481367;Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex;E3bp;Lipoyl-containing pyruvate dehydrogenase complex component X;PDHX;Pdx;Pdx1;Pyruvate dehydrogenase protein X component, mitochondrial

Gene names

Pdhx

Research area

Signal Transduction

Target protein

PDHX

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2416Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.333 2.336 2.295
2.34
800ng/L 1.704 1.701 1.66
1.698
400ng/L 1.165 1.122 1.08
1.078
200ng/L 0.654 0.647 0.606
0.641
100ng/L 0.431 0.423 0.382
0.415
0ng/L 0.052 0.041 0
0.031

Precision

We measured random samples of E2416Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 767.13 33.58 4.38
Intra-Assay 2 18 234.82 12.15 5.18
Intra-Assay 3 18 180.15 13.21 7.33

Protocols

References

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E2416Mo

    $320.00

  • 96T

    E2416Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up