Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2420Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2000ng/L | 2.04 | 2.185 | 2.137 |
| 2.33 | |||
| 1000ng/L | 1.847 | 1.887 | 1.839 |
| 1.928 | |||
| 500ng/L | 1.088 | 1.133 | 1.084 |
| 1.177 | |||
| 250ng/L | 0.647 | 0.661 | 0.613 |
| 0.675 | |||
| 125ng/L | 0.35 | 0.353 | 0.304 |
| 0.355 | |||
| 0ng/L | 0.033 | 0.048 | 0 |
| 0.063 |
Precision
We measured random samples of E2420Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 498.69 | 33.8 | 6.78 |
| Intra-Assay | 2 | 18 | 948.76 | 64.01 | 6.75 |
| Intra-Assay | 3 | 18 | 257.84 | 13.76 | 5.34 |
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