Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2422Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 16mU/ml | 2.157 | 2.101 | 2.052 |
| 2.045 | |||
| 8mU/ml | 1.519 | 1.518 | 1.469 |
| 1.517 | |||
| 4mU/ml | 1.193 | 1.112 | 1.063 |
| 1.031 | |||
| 2mU/ml | 0.659 | 0.665 | 0.616 |
| 0.67 | |||
| 1mU/ml | 0.455 | 0.474 | 0.425 |
| 0.492 | |||
| 0mU/ml | 0.044 | 0.049 | 0 |
| 0.053 |
Precision
We measured random samples of E2422Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 10.49 | 0.61 | 5.81 |
| Intra-Assay | 2 | 18 | 3.54 | 0.29 | 8.2 |
| Intra-Assay | 3 | 18 | 1.17 | 0.09 | 7.81 |
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