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Home > Product > ELISA Kit > Rat Transcription Factor GATA-4, GATA-4 ELISA Kit

Rat Transcription Factor GATA-4, GATA-4 ELISA Kit

Rat Transcription Factor GATA-4, GATA-4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.044ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.044ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Transcription factor GATA-4

Code

E2431Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.044ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (By similarity). In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it downregulates endocardial and endothelial gene expression (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (By similarity). Required during testicular development (By similarity). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (PubMed:15329343). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (PubMed:15329343). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase (By similarity).

UniProt accession

MASS(Da)

44,597

GeneID

54254

Synonyms

DNA-binding protein GATA-GT2;gata4;GATA-binding factor 4;Transcription factor GATA-4

Gene names

Gata4

Research area

Epigenetics and Nuclear Signaling

Target protein

GATA4

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2431Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 1.863 1.909 1.866
1.956
12ng/ml 0.904 1.029 0.985
1.154
6ng/ml 0.472 0.477 0.433
0.482
3ng/ml 0.275 0.275 0.231
0.274
1.5ng/ml 0.164 0.135 0.091
0.105
0ng/ml 0.044 0.044 0
0.043

Precision

We measured random samples of E2431Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.54 0.29 4.4
Intra-Assay 2 18 22.06 0.67 3.05
Intra-Assay 3 18 9.55 0.41 4.24

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2431Ra

    $320.00

  • 96T

    E2431Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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