Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2440Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 800ng/mL | 2.26 | 2.227 | 2.184 |
| 2.193 | |||
| 400ng/mL | 1.134 | 1.206 | 1.163 |
| 1.277 | |||
| 200ng/mL | 0.851 | 0.8 | 0.757 |
| 0.749 | |||
| 100ng/mL | 0.426 | 0.408 | 0.366 |
| 0.39 | |||
| 50ng/mL | 0.179 | 0.178 | 0.135 |
| 0.177 | |||
| 0ng/mL | 0.051 | 0.043 | 0 |
| 0.034 |
Precision
We measured random samples of E2440Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 486.18 | 15.27 | 3.14 |
| Intra-Assay | 2 | 18 | 190.79 | 12.43 | 6.52 |
| Intra-Assay | 3 | 18 | 214.95 | 7.9 | 3.68 |
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