Human Peroxisome Proliferator-activated Receptor Gamma Coactivator 1 Alpha, PGC1A ELISA Kit

Product

OneStep ELISA kit
Primacu ELISA kit
Antibodies
Biochemicals
Recombinant proteins

Service

Custom ELISA assay services
Bulk quote

Research area

Blood coagulation
Metabolism
Cancer
Microbiology
Cardiovascular
Neuroscience
Cell biology
Signal transduction
Developmental biology
Stem cells
Epigenetics and nuclear signaling
Tags & cell markers
Immunology

Resources

Protocols
FAQs
Product brochure
Promotion
Troubleshooting guides
About ELISAs

About us

Company profile
Worldwide distributors
Manufacturing service

Home > Product > ELISA Kit > Human Peroxisome Proliferator-activated Receptor Gamma Coactivator 1 Alpha, PGC1A ELISA Kit

Human Peroxisome Proliferator-activated Receptor Gamma Coactivator 1 Alpha, PGC1A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1 Alpha

Code

E3509Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.021ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.

UniProt accession

Q9UBK2

MASS（Da）

91,027

GeneID

10891

Synonyms

Ligand effect modulator 6;Peroxisome proliferator-activated receptor gamma coactivator 1-alpha;PGC-1-alpha;PPAR-gamma coactivator 1-alpha;PPARGC1A;PPARGC-1-alpha

Gene names

PPARGC1A

Research area

Metabolism

Target protein

PPARGC1A

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E3509Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	1.967	2.045	1.993
	2.124
8ng/ml	1.088	1.017	0.965
	0.946
4ng/ml	0.549	0.514	0.462
	0.479
2ng/ml	0.307	0.242	0.19
	0.177
1ng/ml	0.11	0.113	0.06
	0.116
0ng/ml	0.063	0.052	0
	0.042

Precision

We measured random samples of E3509Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	10.9	0.29	2.7
Intra-Assay	2	18	9.1	0.39	4.4
Intra-Assay	3	18	3.1	0.14	4.6

Protocols

> Protocol Booklet

References

Customer reviews and Q&As

No reviews

Associated products

Datasheet

Size

Code

Price

48T

E3509Hu

$320.00
96T

E3509Hu

$458.00

Add to Cart

Lead time: Within two weeks

Custom quote | Bulk request

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.