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Home > Product > ELISA Kit > Bovine Homeobox Protein Nanog, NANOG ELISA Kit

Bovine Homeobox Protein Nanog, NANOG ELISA Kit

Bovine Homeobox Protein Nanog, NANOG ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 7.98ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 7.98ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Homeobox Protein Nanog

Code

E2272Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

7.98ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator and repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation (By similarity).

UniProt accession

MASS(Da)

34,332

GeneID

538951

Synonyms

Homeobox protein NANOG;Homeobox transcription factor Nanog;NANOG

Gene names

NANOG

Research area

Others

Target protein

NANOG

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2272Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 1.77 1.803 1.761
1.836
800ng/L 1.081 1.007 0.965
0.933
400ng/L 0.624 0.519 0.477
0.414
200ng/L 0.157 0.216 0.174
0.276
100ng/L 0.078 0.107 0.065
0.137
0ng/L 0.044 0.042 0
0.041

Precision

We measured random samples of E2272Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1276.6 41.79 3.3
Intra-Assay 2 18 343.2 15.1 4.4
Intra-Assay 3 18 689.3 21.42 3.1

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 96T

    E2272Bo

    $458.00

  • 48T

    E2272Bo

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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