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Home > Product > ELISA Kit > Mouse Sphingosine-1-phosphate Lyase 1, SGPL1 ELISA Kit

Mouse Sphingosine-1-phosphate Lyase 1, SGPL1 ELISA Kit

Mouse Sphingosine-1-phosphate Lyase 1, SGPL1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.44ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 3.44ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Sphingosine-1-phosphate lyase 1

Code

E2486Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.44ng/L

Detection range

7-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine (PubMed:9464245, PubMed:20097939). Elevates stress-induced ceramide production and apoptosis (PubMed:9464245). Required for global lipid homeostasis in liver and cholesterol homeostasis in fibroblasts (PubMed:20097939, PubMed:28262793). Involved in the regulation of pro-inflammatory response and neutrophil trafficking (PubMed:21173151). Modulates neuronal autophagy via phosphoethanolamine production which regulates accumulation of aggregate-prone proteins such as APP (PubMed:28521611). Seems to play a role in establishing neuronal contact sites and axonal maintenance (By similarity).

UniProt accession

MASS(Da)

63,677

GeneID

20397

Synonyms

AI428538;D10Xrf45;D10Xrf456;mSPL;S1PL;SGPL1;Sphingosine-1-phosphate aldolase;Sphingosine-1-phosphate lyase 1;Spl;SPL 1;SP-lyase 1

Gene names

Sgpl1

Research area

Others

Target protein

SGPL1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2486Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.191 2.147 2.097
2.103
400ng/L 1.365 1.397 1.347
1.429
200ng/L 0.907 0.863 0.813
0.82
100ng/L 0.511 0.532 0.481
0.552
50ng/L 0.268 0.247 0.197
0.226
0ng/L 0.058 0.05 0
0.043

Precision

We measured random samples of E2486Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 456.4 18.58 4.07
Intra-Assay 2 18 193.36 12.16 6.29
Intra-Assay 3 18 159.32 9.08 5.7

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2486Mo

    $320.00

  • 96T

    E2486Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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