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Home > Product > ELISA Kit > Rat Fad-linked Sulfhydryloxidase Alr, GFER ELISA Kit

Rat Fad-linked Sulfhydryloxidase Alr, GFER ELISA Kit

Rat Fad-linked Sulfhydryloxidase Alr, GFER ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 11.57ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 11.57ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

FAD-linked sulfhydryl oxidase ALR

Code

E2486Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

11.57ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. May have a function in liver regeneration and spermatogenesis.

UniProt accession

MASS(Da)

22,837

GeneID

27100

Synonyms

ALR;Augmenter of liver regeneration;FAD-linked sulfhydryl oxidase ALR;GFER

Gene names

Gfer

Research area

Signal Transduction

Target protein

GFER

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2486Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.243 2.296 2.252
2.349
1200ng/L 1.46 1.362 1.318
1.265
600ng/L 0.699 0.721 0.677
0.743
300ng/L 0.385 0.407 0.363
0.429
150ng/L 0.151 0.173 0.129
0.195
0ng/L 0.046 0.044 0
0.042

Precision

We measured random samples of E2486Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 900.94 46.56 5.17
Intra-Assay 2 18 1168.76 42.03 3.6
Intra-Assay 3 18 294.99 14.45 4.9

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2486Ra

    $320.00

  • 96T

    E2486Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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