Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA plate	12 wells * 8 detachable strips
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

These standard curves of E2499Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.029	2.088	2.035
	2.147
12ng/ml	1.421	1.414	1.361
	1.408
6ng/ml	0.914	0.933	0.879
	0.951
3ng/ml	0.528	0.479	0.426
	0.431
1.5ng/ml	0.221	0.265	0.212
	0.31
0ng/ml	0.057	0.054	0
	0.05

We measured random samples of E2499Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	18.77	1.03	5.5
Intra-Assay	2	18	8.37	0.52	6.19
Intra-Assay	3	18	3.31	0.19	5.84

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