Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2522Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 640ng/L | 1.996 | 1.902 | 1.848 |
| 1.807 | |||
| 320ng/L | 0.931 | 0.976 | 0.923 |
| 1.022 | |||
| 160ng/L | 0.511 | 0.524 | 0.471 |
| 0.538 | |||
| 80ng/L | 0.216 | 0.194 | 0.141 |
| 0.172 | |||
| 40ng/L | 0.071 | 0.106 | 0.053 |
| 0.141 | |||
| 0ng/L | 0.052 | 0.053 | 0 |
| 0.054 |
Precision
We measured random samples of E2522Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 477.35 | 15.3 | 3.21 |
| Intra-Assay | 2 | 18 | 363.02 | 13.75 | 3.79 |
| Intra-Assay | 3 | 18 | 150.97 | 6.52 | 4.32 |
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