Mouse Tripartite Motif-containing Protein 72, TRIM72 ELISA Kit

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Home > Product > ELISA Kit > Mouse Tripartite Motif-containing Protein 72, TRIM72 ELISA Kit

Mouse Tripartite Motif-containing Protein 72, TRIM72 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.13ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.13ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Tripartite motif-containing protein 72

Code

E2527Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.13ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca2+-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.

UniProt accession

Q1XH17

MASS（Da）

52,817

GeneID

434246

Synonyms

BC067209;MG53;Mitsugumin-53;TRIM72;Tripartite motif-containing protein 72

Gene names

Trim72

Research area

Signal Transduction

Target protein

TRIM72

Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E2527Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.36	2.225	2.19
	2.09
1200ng/L	1.557	1.584	1.549
	1.611
600ng/L	1.014	1.077	1.042
	1.141
300ng/L	0.71	0.673	0.637
	0.636
150ng/L	0.4	0.404	0.369
	0.408
0ng/L	0.036	0.035	0
	0.035

Precision

We measured random samples of E2527Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	548.92	37.33	6.8
Intra-Assay	2	18	826.2	36.09	4.37
Intra-Assay	3	18	117.13	7.84	6.69

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

48T

E2527Mo

$320.00
96T

E2527Mo

$458.00

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Lead time: Within two weeks

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