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Home > Product > ELISA Kit > Rat Voltage-dependent L-type Calcium Channel Subunit Alpha-1C, CACNA1C ELISA Kit

Rat Voltage-dependent L-type Calcium Channel Subunit Alpha-1C, CACNA1C ELISA Kit

Rat Voltage-dependent L-type Calcium Channel Subunit Alpha-1C, CACNA1C ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.053ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.053ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Voltage-dependent L-type calcium Channel subunit alpha-1C

Code

E2539Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.053ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (Probable) (PubMed:15140941, PubMed:15170217). Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm (By similarity). Plays an important role in excitation-contraction coupling in the heart (By similarity). Required for normal heart development and normal regulation of heart rhythm (By similarity). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (By similarity). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (By similarity).

UniProt accession

MASS(Da)

243,482

GeneID

24239

Synonyms

Cacna1c;Calcium Channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle;Rat brain class C;RATIVS302;RBC;Voltage-dependent L-type calcium Channel subunit alpha-1C;Voltage-gated calcium Channel subunit alpha Cav1.2

Gene names

Cacna1c

Research area

Neuroscience

Target protein

Cacna1c

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2539Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.29 2.227 2.177
2.163
12ng/ml 1.303 1.4 1.35
1.497
6ng/ml 0.812 0.772 0.722
0.733
3ng/ml 0.42 0.409 0.359
0.398
1.5ng/ml 0.227 0.237 0.187
0.248
0ng/ml 0.044 0.05 0
0.057

Precision

We measured random samples of E2539Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 8.45 0.4 4.76
Intra-Assay 2 18 10.7 0.35 3.24
Intra-Assay 3 18 5.42 0.32 5.89

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2539Ra

    $320.00

  • 96T

    E2539Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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