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Home > Product > ELISA Kit > Human Dynein Heavy Chain 5, Axonemal, DNAH5 ELISA Kit

Human Dynein Heavy Chain 5, Axonemal, DNAH5 ELISA Kit

Human Dynein Heavy Chain 5, Axonemal, DNAH5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Dynein Heavy Chain 5,Axonemal

Code

E3543Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.027ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required for structural and functional integrity of the cilia of ependymal cells lining the brain ventricles.

UniProt accession

MASS(Da)

529,021

GeneID

1767

Synonyms

Axonemal beta dynein heavy chain 5;Ciliary dynein heavy chain 5;DNAH 5;DNAH5;Dynein heavy chain 5, axonemal

Gene names

DNAH5

Research area

Signal Transduction

Target protein

DNAH5

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3543Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 1.926 1.834 1.787
1.743
8ng/ml 1.102 1.016 0.969
0.93
4ng/ml 0.462 0.478 0.43
0.493
2ng/ml 0.297 0.22 0.173
0.143
1ng/ml 0.099 0.105 0.057
0.11
0ng/ml 0.06 0.047 0
0.034

Precision

We measured random samples of E3543Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 14.3 0.44 3.1
Intra-Assay 2 18 4.9 0.17 3.4
Intra-Assay 3 18 9.5 0.44 4.6

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

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