Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2547Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 640ng/L | 2.056 | 2.185 | 2.141 |
| 2.314 | |||
| 320ng/L | 1.772 | 1.753 | 1.709 |
| 1.735 | |||
| 160ng/L | 1.204 | 1.23 | 1.186 |
| 1.256 | |||
| 80ng/L | 0.609 | 0.647 | 0.602 |
| 0.684 | |||
| 40ng/L | 0.422 | 0.39 | 0.346 |
| 0.358 | |||
| 0ng/L | 0.028 | 0.044 | 0 |
| 0.06 |
Precision
We measured random samples of E2547Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 267.34 | 14.64 | 5.48 |
| Intra-Assay | 2 | 18 | 133.28 | 8.57 | 6.43 |
| Intra-Assay | 3 | 18 | 77.86 | 5 | 6.42 |
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