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Home > Product > ELISA Kit > Mouse Twist-related Protein 1, TWIST1 ELISA Kit

Mouse Twist-related Protein 1, TWIST1 ELISA Kit

Mouse Twist-related Protein 1, TWIST1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.63ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 5.63ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Twist-related protein 1

Code

E2547Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.63ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-ARNTL/BMAL1 heterodimer.

UniProt accession

MASS(Da)

21,198

GeneID

22160

Synonyms

bHLHa;bHLHa38;M-Twi;M-Twist;pd;Pde;pdt;Pluri;Ska;Ska;Twist;TWIST1;Twist-related protein 1

Gene names

Twist1

Research area

Neuroscience

Target protein

TWIST1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2547Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.293 2.279 2.237
2.264
600ng/L 1.47 1.446 1.404
1.421
300ng/L 0.545 0.654 0.613
0.764
150ng/L 0.441 0.43 0.388
0.419
75ng/L 0.172 0.183 0.142
0.195
0ng/L 0.035 0.041 0
0.048

Precision

We measured random samples of E2547Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 244.29 10.93 4.48
Intra-Assay 2 18 340.99 16.93 4.97
Intra-Assay 3 18 536.52 18.72 3.49

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2547Mo

    $320.00

  • 96T

    E2547Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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