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Home > Product > ELISA Kit > Mouse Ubiquitin-like Modifier-activating Enzyme 1, UBA1 ELISA Kit

Mouse Ubiquitin-like Modifier-activating Enzyme 1, UBA1 ELISA Kit

Mouse Ubiquitin-like Modifier-activating Enzyme 1, UBA1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.66ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 1.66ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Ubiquitin-like modifier-activating enzyme 1

Code

E2551Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.66ng/L

Detection range

3-900ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1511901). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.

UniProt accession

MASS(Da)

117,809

GeneID

22201

Synonyms

A1;A1S9;Sb;Sbx;UBA1;Ube-1;Ube1x;Ubiquitin-activating enzyme E1;Ubiquitin-activating enzyme E1 X;Ubiquitin-like modifier-activating enzyme 1;Ubiquitin-like modifier-activating enzyme 1 X

Gene names

Uba1

Research area

Cell Biology

Target protein

UBA1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2551Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480ng/L 2.151 2.125 2.083
2.1
240ng/L 1.427 1.493 1.45
1.558
120ng/L 0.581 0.641 0.599
0.702
60ng/L 0.347 0.391 0.349
0.436
30ng/L 0.204 0.182 0.14
0.161
0ng/L 0.038 0.043 0
0.048

Precision

We measured random samples of E2551Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 259.8 8.5 3.27
Intra-Assay 2 18 107.76 4.54 4.21
Intra-Assay 3 18 178.06 7.39 4.15

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2551Mo

    $320.00

  • 96T

    E2551Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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