Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2553Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 800ng/L | 2.142 | 2.221 | 2.183 |
| 2.299 | |||
| 400ng/L | 1.464 | 1.39 | 1.353 |
| 1.316 | |||
| 200ng/L | 0.848 | 0.787 | 0.75 |
| 0.727 | |||
| 100ng/L | 0.504 | 0.508 | 0.47 |
| 0.511 | |||
| 50ng/L | 0.232 | 0.271 | 0.233 |
| 0.31 | |||
| 0ng/L | 0.035 | 0.038 | 0 |
| 0.04 |
Precision
We measured random samples of E2553Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 309.83 | 16.33 | 5.27 |
| Intra-Assay | 2 | 18 | 535.67 | 19.84 | 3.7 |
| Intra-Assay | 3 | 18 | 166.44 | 12.41 | 7.46 |
Protocols
References
Customer reviews and Q&As
Associated products
