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Home > Product > ELISA Kit > Rat Voltage-dependent L-type Calcium Channel Subunit Alpha-1D, CACNA1D ELISA Kit

Rat Voltage-dependent L-type Calcium Channel Subunit Alpha-1D, CACNA1D ELISA Kit

Rat Voltage-dependent L-type Calcium Channel Subunit Alpha-1D, CACNA1D ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.03ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 3.03ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Voltage-dependent L-type calcium Channel subunit alpha-1D

Code

E2558Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.03ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, and by benzothiazepines.

UniProt accession

MASS(Da)

250,136

GeneID

29716

Synonyms

Cacna1d;Calcium Channel, L type, alpha-1 polypeptide, isoform 2;Rat brain class D;RBD;Voltage-dependent L-type calcium Channel subunit alpha-1D;Voltage-gated calcium Channel subunit alpha Cav1.3

Gene names

Cacna1d

Research area

Neuroscience

Target protein

Cacna1d

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2558Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.24 2.293 2.235
2.345
600ng/L 1.417 1.354 1.297
1.291
300ng/L 0.706 0.732 0.675
0.759
150ng/L 0.532 0.5 0.443
0.468
75ng/L 0.243 0.269 0.211
0.294
0ng/L 0.066 0.057 0
0.049

Precision

We measured random samples of E2558Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 608.72 20.7 3.4
Intra-Assay 2 18 222.32 12.18 5.48
Intra-Assay 3 18 323.53 20.95 6.47

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2558Ra

    $320.00

  • 96T

    E2558Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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