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Home > Product > ELISA Kit > Mouse Vinculin, VCL ELISA Kit

Mouse Vinculin, VCL ELISA Kit

Mouse Vinculin, VCL ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.63ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.63ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Vinculin

Code

E2563Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.63ng/L

Detection range

20-3500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity).

UniProt accession

MASS(Da)

116,717

GeneID

22330

Synonyms

9430097D22;AA571387;AI462105;AW545629;Metavinculin;VCL;Vinculin

Gene names

Vcl

Research area

Signal Transduction

Target protein

VCL

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2563Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 1.717 1.824 1.795
1.932
1000ng/L 0.886 0.895 0.865
0.903
500ng/L 0.585 0.548 0.519
0.511
250ng/L 0.179 0.197 0.168
0.215
125ng/L 0.141 0.108 0.079
0.075
0ng/L 0.032 0.029 0
0.027

Precision

We measured random samples of E2563Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 797.43 38.98 4.89
Intra-Assay 2 18 1893.59 45.16 2.38
Intra-Assay 3 18 726.3 41.02 5.65

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2563Mo

    $320.00

  • 96T

    E2563Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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