Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2565Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 2.428 | 2.292 | 2.228 |
| 2.155 | |||
| 800ng/L | 1.469 | 1.375 | 1.311 |
| 1.281 | |||
| 400ng/L | 0.855 | 0.842 | 0.779 |
| 0.83 | |||
| 200ng/L | 0.476 | 0.459 | 0.395 |
| 0.442 | |||
| 100ng/L | 0.243 | 0.242 | 0.178 |
| 0.24 | |||
| 0ng/L | 0.052 | 0.064 | 0 |
| 0.075 |
Precision
We measured random samples of E2565Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 970.38 | 43.54 | 4.49 |
| Intra-Assay | 2 | 18 | 501.01 | 27.47 | 5.48 |
| Intra-Assay | 3 | 18 | 251.02 | 15.06 | 6 |
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