Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2572Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2000ng/L | 2.078 | 2.092 | 2.044 |
| 2.105 | |||
| 1000ng/L | 1.203 | 1.216 | 1.168 |
| 1.228 | |||
| 500ng/L | 0.79 | 0.822 | 0.774 |
| 0.853 | |||
| 250ng/L | 0.446 | 0.486 | 0.438 |
| 0.527 | |||
| 125ng/L | 0.203 | 0.223 | 0.176 |
| 0.244 | |||
| 0ng/L | 0.048 | 0.048 | 0 |
| 0.048 |
Precision
We measured random samples of E2572Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1547.03 | 66.04 | 4.27 |
| Intra-Assay | 2 | 18 | 314.55 | 16.29 | 5.18 |
| Intra-Assay | 3 | 18 | 527.47 | 39.17 | 7.43 |
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