Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2613Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1000ng/L | 2.304 | 2.244 | 2.195 |
| 2.184 | |||
| 500ng/L | 1.779 | 1.847 | 1.797 |
| 1.915 | |||
| 250ng/L | 1.096 | 1.118 | 1.069 |
| 1.14 | |||
| 125ng/L | 0.741 | 0.728 | 0.679 |
| 0.716 | |||
| 62.5ng/L | 0.438 | 0.411 | 0.361 |
| 0.384 | |||
| 0ng/L | 0.047 | 0.049 | 0 |
| 0.052 |
Precision
We measured random samples of E2613Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 78.97 | 5.06 | 6.41 |
| Intra-Assay | 2 | 18 | 311.55 | 12.39 | 3.98 |
| Intra-Assay | 3 | 18 | 136.21 | 7.06 | 5.19 |
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