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Home > Product > ELISA Kit > Mouse Autophagy Related Protein 4A, ATG4A ELISA Kit

Mouse Autophagy Related Protein 4A, ATG4A ELISA Kit

Mouse Autophagy Related Protein 4A, ATG4A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 12.58ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 12.58ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Autophagy related protein 4A

Code

E2635Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

12.58ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms (By similarity).

UniProt accession

MASS(Da)

45,095

GeneID

666468

Synonyms

AI627006;Ap;Apg4a;ATG4A;Atg4al;Aut;Autl2;AUT-like 2 cysteine endopeptidase;Autophagin-2;Autophagy-related cysteine endopeptidase 2;Autophagy-related protein 4 homolog A;Cysteine protease ATG4A

Gene names

Atg4a

Research area

Cancer

Target protein

ATG4A

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2635Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4000ng/L 2.142 2.115 2.075
2.088
2000ng/L 1.731 1.737 1.698
1.744
1000ng/L 1.042 1.067 1.027
1.092
500ng/L 0.66 0.687 0.647
0.713
250ng/L 0.398 0.401 0.361
0.404
0ng/L 0.051 0.04 0
0.028

Precision

We measured random samples of E2635Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 818.76 50.58 6.18
Intra-Assay 2 18 1360.6 67.77 4.98
Intra-Assay 3 18 528.2 37.54 7.11

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2635Mo

    $320.00

  • 96T

    E2635Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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