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Home > Product > ELISA Kit > Mouse Autophagy Related Protein 13, ATG13 ELISA Kit

Mouse Autophagy Related Protein 13, ATG13 ELISA Kit

Mouse Autophagy Related Protein 13, ATG13 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.78ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 2.78ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Autophagy related protein 13

Code

E2645Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.78ng/L

Detection range

5-1000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation.

UniProt accession

MASS(Da)

56,441

GeneID

51897

Synonyms

1110053A20Rik;ATG13;Autophagy-related protein 13;D2Ertd391;D2Ertd391e;Harbi1

Gene names

Atg13

Research area

Cancer; Metabolism

Target protein

ATG13

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2645Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/L 2.549 2.593 2.565
2.638
320ng/L 1.375 1.397 1.368
1.418
160ng/L 0.848 0.885 0.856
0.921
80ng/L 0.425 0.447 0.418
0.469
40ng/L 0.239 0.221 0.192
0.204
0ng/L 0.029 0.029 0
0.028

Precision

We measured random samples of E2645Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 414.18 14.76 3.56
Intra-Assay 2 18 131.95 9.34 7.08
Intra-Assay 3 18 155.47 7.74 4.98

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2645Mo

    $320.00

  • 96T

    E2645Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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