Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2680Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 2.097 | 2.14 | 2.093 |
| 2.184 | |||
| 1200ng/L | 1.912 | 1.804 | 1.757 |
| 1.696 | |||
| 600ng/L | 1.263 | 1.262 | 1.214 |
| 1.261 | |||
| 300ng/L | 0.668 | 0.701 | 0.653 |
| 0.733 | |||
| 150ng/L | 0.394 | 0.403 | 0.355 |
| 0.411 | |||
| 0ng/L | 0.038 | 0.048 | 0 |
| 0.057 |
Precision
We measured random samples of E2680Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 739.08 | 42.62 | 5.77 |
| Intra-Assay | 2 | 18 | 200.79 | 11.14 | 5.55 |
| Intra-Assay | 3 | 18 | 538.3 | 28.17 | 5.23 |
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