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Home > Product > ELISA Kit > Human Exostoses 1, EXT1 ELISA Kit

Human Exostoses 1, EXT1 ELISA Kit

Human Exostoses 1, EXT1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Exostoses 1

Code

E2692Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.024ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Exostosin-1 is a protein that in humans is encoded by the EXT1 gene. This gene encodes one of the two endoplasmic reticulum-resident type II transmembrane glycosyltransferase – the other being EXT2 – which are involved in the chain elongation step of heparan sulfate biosynthesis. Mutations in this gene cause the type I form of Multiple Exostoses.

UniProt accession

MASS(Da)

74,697

GeneID

2134

Synonyms

Exostosin-L;Exostosin-like 1;EXT1;EXTL;Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase;Multiple exostosis-like protein

Gene names

EXTL1

Research area

Others

Target protein

EXT1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2692Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 1.997 1.971 1.931
1.946
8ng/ml 1.113 1.034 0.993
0.955
4ng/ml 0.471 0.544 0.503
0.617
2ng/ml 0.179 0.17 0.129
0.161
1ng/ml 0.149 0.116 0.075
0.082
0ng/ml 0.051 0.04 0
0.03

Precision

We measured random samples of E2692Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.62 0.16 4.4
Intra-Assay 2 18 9.82 0.22 2.27
Intra-Assay 3 18 8.32 0.28 3.36

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2692Hu

    $320.00

  • 96T

    E2692Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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