Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2697Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 2.051 | 2.129 | 2.084 |
| 2.207 | |||
| 600ng/L | 1.713 | 1.679 | 1.634 |
| 1.645 | |||
| 300ng/L | 0.995 | 1.039 | 0.995 |
| 1.083 | |||
| 150ng/L | 0.721 | 0.681 | 0.637 |
| 0.641 | |||
| 75ng/L | 0.488 | 0.453 | 0.409 |
| 0.418 | |||
| 0ng/L | 0.028 | 0.045 | 0 |
| 0.062 |
Precision
We measured random samples of E2697Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 660.46 | 37.6 | 5.69 |
| Intra-Assay | 2 | 18 | 110.64 | 8.42 | 7.61 |
| Intra-Assay | 3 | 18 | 228.49 | 15.23 | 6.66 |
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