Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2707Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 2.243 | 2.188 | 2.142 |
| 2.132 | |||
| 1200ng/L | 1.351 | 1.345 | 1.299 |
| 1.339 | |||
| 600ng/L | 0.77 | 0.836 | 0.791 |
| 0.903 | |||
| 300ng/L | 0.467 | 0.472 | 0.426 |
| 0.476 | |||
| 150ng/L | 0.303 | 0.269 | 0.223 |
| 0.235 | |||
| 0ng/L | 0.036 | 0.046 | 0 |
| 0.055 |
Precision
We measured random samples of E2707Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1455.94 | 58.96 | 4.05 |
| Intra-Assay | 2 | 18 | 738.36 | 44.95 | 6.09 |
| Intra-Assay | 3 | 18 | 370.76 | 22.08 | 5.96 |
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