Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2745Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2000ng/L | 2.146 | 2.264 | 2.199 |
| 2.383 | |||
| 1000ng/L | 1.25 | 1.329 | 1.263 |
| 1.407 | |||
| 500ng/L | 0.804 | 0.814 | 0.748 |
| 0.823 | |||
| 250ng/L | 0.544 | 0.529 | 0.463 |
| 0.514 | |||
| 125ng/L | 0.257 | 0.255 | 0.189 |
| 0.252 | |||
| 0ng/L | 0.062 | 0.066 | 0 |
| 0.069 |
Precision
We measured random samples of E2745Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 462.25 | 34.05 | 7.37 |
| Intra-Assay | 2 | 18 | 930.82 | 36.42 | 3.91 |
| Intra-Assay | 3 | 18 | 618.02 | 37.56 | 6.08 |
Protocols
References
Customer reviews and Q&As
Associated products
