Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2759Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 120ng/ml | 2.048 | 2.124 | 2.091 |
| 2.2 | |||
| 60ng/ml | 1.597 | 1.61 | 1.577 |
| 1.623 | |||
| 30ng/ml | 0.958 | 1.013 | 0.98 |
| 1.067 | |||
| 15ng/ml | 0.641 | 0.693 | 0.66 |
| 0.745 | |||
| 7.5ng/ml | 0.446 | 0.463 | 0.431 |
| 0.481 | |||
| 0ng/ml | 0.039 | 0.033 | 0 |
| 0.027 |
Precision
We measured random samples of E2759Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 5.47 | 0.4 | 7.22 |
| Intra-Assay | 2 | 18 | 42.51 | 1.79 | 4.22 |
| Intra-Assay | 3 | 18 | 25.48 | 1.62 | 6.37 |
Protocols
References
Customer reviews and Q&As
Associated products
