Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2775Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 16ng/ml | 2.104 | 2.195 | 2.157 |
| 2.287 | |||
| 8ng/ml | 1.755 | 1.733 | 1.695 |
| 1.712 | |||
| 4ng/ml | 1.018 | 1.036 | 0.997 |
| 1.055 | |||
| 2ng/ml | 0.769 | 0.766 | 0.727 |
| 0.763 | |||
| 1ng/ml | 0.443 | 0.429 | 0.39 |
| 0.416 | |||
| 0ng/ml | 0.029 | 0.039 | 0 |
| 0.048 |
Precision
We measured random samples of E2775Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 8.7 | 0.45 | 5.16 |
| Intra-Assay | 2 | 18 | 1.33 | 0.09 | 6.62 |
| Intra-Assay | 3 | 18 | 2.23 | 0.14 | 6.07 |
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