Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2794Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 48ng/ml | 2.28 | 2.19 | 2.152 |
| 2.1 | |||
| 24ng/ml | 1.468 | 1.388 | 1.35 |
| 1.308 | |||
| 12ng/ml | 0.953 | 0.898 | 0.86 |
| 0.843 | |||
| 6ng/ml | 0.439 | 0.496 | 0.458 |
| 0.552 | |||
| 3ng/ml | 0.235 | 0.232 | 0.194 |
| 0.229 | |||
| 0ng/ml | 0.04 | 0.038 | 0 |
| 0.036 |
Precision
We measured random samples of E2794Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 23.8 | 1.12 | 4.7 |
| Intra-Assay | 2 | 18 | 10.1 | 0.57 | 5.64 |
| Intra-Assay | 3 | 18 | 14.47 | 0.66 | 4.55 |
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