Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2819Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 8ng/ml | 1.812 | 1.904 | 1.846 |
| 1.997 | |||
| 4ng/ml | 1.149 | 1.145 | 1.087 |
| 1.141 | |||
| 2ng/ml | 0.495 | 0.534 | 0.476 |
| 0.574 | |||
| 1ng/ml | 0.299 | 0.25 | 0.192 |
| 0.2 | |||
| 0.5ng/ml | 0.122 | 0.133 | 0.075 |
| 0.144 | |||
| 0ng/ml | 0.051 | 0.058 | 0 |
| 0.064 |
Precision
We measured random samples of E2819Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 4.52 | 0.18 | 3.92 |
| Intra-Assay | 2 | 18 | 3.26 | 0.15 | 4.66 |
| Intra-Assay | 3 | 18 | 2.13 | 0.08 | 3.82 |
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