Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2852Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 2.408 | 2.464 | 2.424 |
| 2.521 | |||
| 1200ng/L | 1.396 | 1.497 | 1.457 |
| 1.598 | |||
| 600ng/L | 0.763 | 0.773 | 0.732 |
| 0.783 | |||
| 300ng/L | 0.423 | 0.433 | 0.393 |
| 0.444 | |||
| 150ng/L | 0.201 | 0.22 | 0.18 |
| 0.24 | |||
| 0ng/L | 0.04 | 0.04 | 0 |
| 0.04 |
Precision
We measured random samples of E2852Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1240.29 | 36.75 | 2.96 |
| Intra-Assay | 2 | 18 | 392.16 | 19.3 | 4.92 |
| Intra-Assay | 3 | 18 | 604.84 | 25.61 | 4.23 |
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