Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2854Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 800ng/L | 1.952 | 2.032 | 2.003 |
| 2.113 | |||
| 400ng/L | 0.981 | 1.056 | 1.027 |
| 1.131 | |||
| 200ng/L | 0.483 | 0.432 | 0.403 |
| 0.382 | |||
| 100ng/L | 0.197 | 0.228 | 0.199 |
| 0.258 | |||
| 50ng/L | 0.13 | 0.129 | 0.1 |
| 0.128 | |||
| 0ng/L | 0.028 | 0.029 | 0 |
| 0.03 |
Precision
We measured random samples of E2854Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 190.2 | 9.51 | 5 |
| Intra-Assay | 2 | 18 | 434.79 | 16.48 | 3.79 |
| Intra-Assay | 3 | 18 | 696.16 | 23.7 | 3.4 |
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