Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2862Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 40ng/ml | 2 | 1.971 | 1.937 |
| 1.943 | |||
| 20ng/ml | 1.381 | 1.437 | 1.403 |
| 1.493 | |||
| 10ng/ml | 0.663 | 0.705 | 0.671 |
| 0.747 | |||
| 5ng/ml | 0.33 | 0.343 | 0.309 |
| 0.357 | |||
| 2.5ng/ml | 0.211 | 0.211 | 0.177 |
| 0.212 | |||
| 0ng/ml | 0.045 | 0.034 | 0 |
| 0.023 |
Precision
We measured random samples of E2862Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 12.13 | 0.31 | 2.58 |
| Intra-Assay | 2 | 18 | 24.82 | 1.2 | 4.84 |
| Intra-Assay | 3 | 18 | 7.26 | 0.29 | 3.94 |
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