Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2870Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 2.132 | 2.201 | 2.148 |
| 2.271 | |||
| 1200ng/L | 1.382 | 1.372 | 1.318 |
| 1.362 | |||
| 600ng/L | 0.572 | 0.595 | 0.542 |
| 0.618 | |||
| 300ng/L | 0.38 | 0.355 | 0.301 |
| 0.33 | |||
| 150ng/L | 0.202 | 0.163 | 0.11 |
| 0.125 | |||
| 0ng/L | 0.048 | 0.054 | 0 |
| 0.059 |
Precision
We measured random samples of E2870Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 794.44 | 33.2 | 4.18 |
| Intra-Assay | 2 | 18 | 1191.41 | 35.55 | 2.98 |
| Intra-Assay | 3 | 18 | 529.02 | 24.61 | 4.65 |
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