Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2934Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 400ng/L | 2.234 | 2.197 | 2.134 |
| 2.16 | |||
| 200ng/L | 1.171 | 1.162 | 1.098 |
| 1.152 | |||
| 100ng/L | 0.69 | 0.699 | 0.636 |
| 0.708 | |||
| 50ng/L | 0.392 | 0.415 | 0.351 |
| 0.437 | |||
| 25ng/L | 0.231 | 0.239 | 0.176 |
| 0.248 | |||
| 0ng/L | 0.063 | 0.063 | 0 |
| 0.064 |
Precision
We measured random samples of E2934Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 317.17 | 9.84 | 3.1 |
| Intra-Assay | 2 | 18 | 175.09 | 8.58 | 4.9 |
| Intra-Assay | 3 | 18 | 79.89 | 4.35 | 5.44 |
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