Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2937Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 12ng/ml | 1.994 | 2.106 | 2.059 |
| 2.218 | |||
| 6ng/ml | 1.304 | 1.373 | 1.326 |
| 1.442 | |||
| 3ng/ml | 0.583 | 0.67 | 0.623 |
| 0.757 | |||
| 1.5ng/ml | 0.387 | 0.377 | 0.329 |
| 0.366 | |||
| 0.75ng/ml | 0.153 | 0.167 | 0.12 |
| 0.18 | |||
| 0ng/ml | 0.05 | 0.047 | 0 |
| 0.045 |
Precision
We measured random samples of E2937Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 3.23 | 0.15 | 4.56 |
| Intra-Assay | 2 | 18 | 8.38 | 0.35 | 4.23 |
| Intra-Assay | 3 | 18 | 1.79 | 0.07 | 3.88 |
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