Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2961Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 24ng/mL | 2.35 | 2.368 | 2.322 |
| 2.385 | |||
| 12ng/mL | 1.355 | 1.324 | 1.278 |
| 1.292 | |||
| 6ng/mL | 0.662 | 0.674 | 0.628 |
| 0.686 | |||
| 3ng/mL | 0.354 | 0.336 | 0.29 |
| 0.318 | |||
| 1.5ng/mL | 0.171 | 0.154 | 0.108 |
| 0.137 | |||
| 0ng/mL | 0.053 | 0.046 | 0 |
| 0.039 |
Precision
We measured random samples of E2961Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 13.26 | 0.36 | 2.73 |
| Intra-Assay | 2 | 18 | 6.06 | 0.28 | 4.63 |
| Intra-Assay | 3 | 18 | 7.18 | 0.29 | 4.09 |
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