Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2968Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 6.4ng/ml | 2.158 | 2.178 | 2.123 |
| 2.197 | |||
| 3.2ng/ml | 1.721 | 1.645 | 1.591 |
| 1.57 | |||
| 1.6ng/ml | 0.978 | 0.999 | 0.944 |
| 1.02 | |||
| 0.8ng/ml | 0.669 | 0.715 | 0.66 |
| 0.761 | |||
| 0.4ng/ml | 0.415 | 0.429 | 0.374 |
| 0.442 | |||
| 0ng/ml | 0.047 | 0.055 | 0 |
| 0.063 |
Precision
We measured random samples of E2968Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1.04 | 0.06 | 6.13 |
| Intra-Assay | 2 | 18 | 2.31 | 0.08 | 3.61 |
| Intra-Assay | 3 | 18 | 0.75 | 0.06 | 7.79 |
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