Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E3064Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 120ng/mL | 2.321 | 2.255 | 2.205 |
| 2.189 | |||
| 60ng/mL | 1.658 | 1.593 | 1.543 |
| 1.528 | |||
| 30ng/mL | 1.002 | 0.98 | 0.929 |
| 0.958 | |||
| 15ng/mL | 0.753 | 0.758 | 0.708 |
| 0.762 | |||
| 7.5ng/mL | 0.493 | 0.449 | 0.399 |
| 0.406 | |||
| 0ng/mL | 0.047 | 0.05 | 0 |
| 0.053 |
Precision
We measured random samples of E3064Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 6.77 | 0.69 | 10.17 |
| Intra-Assay | 2 | 18 | 18.44 | 1.16 | 6.27 |
| Intra-Assay | 3 | 18 | 64.51 | 2.24 | 3.48 |
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