Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E3065Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 80ng/ml | 2.074 | 2.08 | 2.041 |
| 2.087 | |||
| 40ng/ml | 1.351 | 1.364 | 1.324 |
| 1.376 | |||
| 20ng/ml | 0.759 | 0.79 | 0.751 |
| 0.822 | |||
| 10ng/ml | 0.438 | 0.442 | 0.403 |
| 0.446 | |||
| 5ng/ml | 0.224 | 0.237 | 0.198 |
| 0.251 | |||
| 0ng/ml | 0.035 | 0.039 | 0 |
| 0.044 |
Precision
We measured random samples of E3065Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 34.09 | 2.04 | 5.97 |
| Intra-Assay | 2 | 18 | 44.82 | 1.64 | 3.67 |
| Intra-Assay | 3 | 18 | 17.96 | 1.24 | 6.91 |
Protocols
References
Customer reviews and Q&As
Associated products
