Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E3088Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 2.109 | 2.07 | 2.018 |
| 2.031 | |||
| 800ng/L | 1.626 | 1.626 | 1.574 |
| 1.627 | |||
| 400ng/L | 1.073 | 1.081 | 1.03 |
| 1.09 | |||
| 200ng/L | 0.749 | 0.718 | 0.666 |
| 0.688 | |||
| 100ng/L | 0.477 | 0.484 | 0.432 |
| 0.492 | |||
| 0ng/L | 0.051 | 0.052 | 0 |
| 0.053 |
Precision
We measured random samples of E3088Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 141.09 | 9.63 | 6.83 |
| Intra-Assay | 2 | 18 | 470.35 | 39.44 | 8.39 |
| Intra-Assay | 3 | 18 | 887.79 | 51.69 | 5.82 |
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