Human 6-phosphofructo-2-kinase, Fructose-2, 6-biphosphatase 2, PFKFB2 ELISA Kit

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Home > Product > ELISA Kit > Human 6-phosphofructo-2-kinase, Fructose-2, 6-biphosphatase 2, PFKFB2 ELISA Kit

Human 6-phosphofructo-2-kinase, Fructose-2, 6-biphosphatase 2, PFKFB2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.017ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.017ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2

Code

E3191Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.017ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 is an enzyme that in humans is encoded by the PFKFB2 gene. The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene.

UniProt accession

O60825

MASS（Da）

58,477

GeneID

5208

Synonyms

6PF-2-K/Fru-2,6-P2ase 2;6PF-2-K/Fru-2,6-P2ase heart-type isozyme [Includes: 6-phosphofructo-2-kinase;6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2;Fructose-2,6-bisphosphatase;PFK/FBPase 2;PFK-2/FBPase-2;PFKFB2

Gene names

PFKFB2

Research area

Others

Target protein

PFKFB2

Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E3191Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.271	2.264	2.195
	2.258
4ng/ml	1.316	1.389	1.319
	1.461
2ng/ml	0.773	0.788	0.719
	0.803
1ng/ml	0.478	0.507	0.438
	0.536
0.5ng/ml	0.258	0.29	0.221
	0.322
0ng/ml	0.067	0.069	0
	0.072

Precision

We measured random samples of E3191Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.13	0.2	6.29
Intra-Assay	2	18	4.34	0.17	4.01
Intra-Assay	3	18	1.71	0.14	7.99

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

48T

E3191Hu

$320.00
96T

E3191Hu

$458.00

Add to Cart

Lead time: Within two weeks

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