Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E3231Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 640ng/L | 1.723 | 1.847 | 1.805 |
| 1.972 | |||
| 320ng/L | 1.106 | 1.039 | 0.997 |
| 0.972 | |||
| 160ng/L | 0.453 | 0.47 | 0.428 |
| 0.488 | |||
| 80ng/L | 0.283 | 0.284 | 0.241 |
| 0.284 | |||
| 40ng/L | 0.13 | 0.134 | 0.091 |
| 0.137 | |||
| 0ng/L | 0.036 | 0.043 | 0 |
| 0.049 |
Precision
We measured random samples of E3231Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 476.02 | 14.2 | 2.98 |
| Intra-Assay | 2 | 18 | 272.05 | 11.54 | 4.24 |
| Intra-Assay | 3 | 18 | 164.43 | 7.5 | 4.56 |
Protocols
References (4)
Customer reviews and Q&As
