Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E3249Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 4000ng/L | 1.813 | 1.943 | 1.903 |
| 2.072 | |||
| 2000ng/L | 0.95 | 0.973 | 0.934 |
| 0.997 | |||
| 1000ng/L | 0.49 | 0.478 | 0.438 |
| 0.465 | |||
| 500ng/L | 0.257 | 0.27 | 0.23 |
| 0.282 | |||
| 250ng/L | 0.072 | 0.085 | 0.046 |
| 0.099 | |||
| 0ng/L | 0.05 | 0.04 | 0 |
| 0.03 |
Precision
We measured random samples of E3249Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 3011.21 | 82.94 | 2.75 |
| Intra-Assay | 2 | 18 | 1079.04 | 57.57 | 5.34 |
| Intra-Assay | 3 | 18 | 2173.84 | 80.77 | 3.72 |
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