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Home > Product > ELISA Kit > Human Neurogenic Locus Notch Homolog Protein 3, NOTCH3 ELISA Kit

Human Neurogenic Locus Notch Homolog Protein 3, NOTCH3 ELISA Kit

Human Neurogenic Locus Notch Homolog Protein 3, NOTCH3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 36.17ng/L

Detection range: 70-15000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 36.17ng/L

Detection range: 70-15000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Neurogenic locus notch homolog protein 3

Code

E3263Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

36.17ng/L

Detection range

70-15000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination (PubMed:15350543). Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity).

UniProt accession

MASS(Da)

243,631

GeneID

4854

Synonyms

CADASIL;CADASIL1;CASIL;IMF2;LMNS;Neurogenic locus notch homolog protein 3;Notch 3;Notch 3 extracellular truncation;Notch 3 intracellular domain];NOTCH3

Gene names

NOTCH3

Research area

Neuroscience

Target protein

NOTCH3

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3263Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8000ng/L 2.106 2.194 2.151
2.281
4000ng/L 1.537 1.647 1.604
1.756
2000ng/L 0.974 1.04 0.998
1.107
1000ng/L 0.701 0.697 0.655
0.694
500ng/L 0.428 0.449 0.406
0.47
0ng/L 0.04 0.043 0
0.045

Precision

We measured random samples of E3263Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2001.48 75.45 3.77
Intra-Assay 2 18 2810.11 106.45 3.79
Intra-Assay 3 18 455.16 29.79 6.54

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E3263Hu

    $320.00

  • 96T

    E3263Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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