Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E3362Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 40ng/ml | 2.062 | 2.088 | 2.045 |
| 2.114 | |||
| 20ng/ml | 1.381 | 1.368 | 1.325 |
| 1.355 | |||
| 10ng/ml | 0.674 | 0.7 | 0.658 |
| 0.726 | |||
| 5ng/ml | 0.442 | 0.38 | 0.337 |
| 0.319 | |||
| 2.5ng/ml | 0.196 | 0.182 | 0.139 |
| 0.167 | |||
| 0ng/ml | 0.036 | 0.043 | 0 |
| 0.05 |
Precision
We measured random samples of E3362Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 26.19 | 0.96 | 3.66 |
| Intra-Assay | 2 | 18 | 5.46 | 0.27 | 4.9 |
| Intra-Assay | 3 | 18 | 14.35 | 0.71 | 4.94 |
Protocols
References
Customer reviews and Q&As
Associated products
